Zonulin Family Peptide Test (Stool)

Assess Intestinal Barrier Function (LEAKY GUT)

The Zonulin Family Peptide (ZFP) Stool Test measures levels of zonulin-related proteins in stool, which are markers associated with the regulation of intestinal permeability.

Zonulin plays an important role in controlling the tight junctions between intestinal cells. When zonulin levels increase, these tight junctions can loosen, allowing substances such as toxins, microbes, and partially digested food particles to pass through the intestinal barrier more easily. This process is commonly referred to as increased intestinal permeability or “leaky gut.”

Measuring zonulin levels can help evaluate gut barrier integrity and identify whether intestinal permeability may be contributing to digestive symptoms or systemic inflammation.

Why is this test important

Disruption of the intestinal barrier has been associated with a range of health concerns, including:

• Chronic digestive symptoms
• Food sensitivities
• Inflammatory conditions
• Autoimmune activation
• Gut microbiome imbalances
• Metabolic dysfunction

Identifying increased intestinal permeability can help guide targeted gut-healing and microbiome restoration strategies.

Test Highlights

✔ Evaluates intestinal permeability and gut barrier integrity
✔ Non-invasive stool-based test
✔ Helps identify potential leaky gut–related inflammation
✔ Useful in investigating chronic digestive or inflammatory symptoms

Sample Required

Stool sample

Detailed sample collection instructions will be provided after booking the test.

Who should consider this test?

This test may be useful for individuals experiencing:

• Persistent inflammation
• Chronic bloating or digestive discomfort
• Food sensitivities or intolerances
• Autoimmune conditions
• Gut microbiome imbalances
• Unexplained fatigue or systemic symptoms linked to gut health

Turnaround Time

Typically 4 weeks after sample submission.